islet cells Search Results


ia2  (Proteintech)
92
Proteintech ia2
Ia2, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/islet+cells/IA-2%2FPTPRN+Antibody/pmc06851310-608-42-43
Average 92 stars, based on 1 article reviews
ia2 - by Bioz Stars, 2026-09
92/100 stars
  Buy from Supplier
sox13  (Proteintech)
93
Proteintech sox13
POU3F2 activates expression of <t>SOX13</t> and ADNP to regulate canonical Wnt signalling in NPCs. ( A ) Potential direct effectors of POU3F2 were prioritized if they belonged to the TCF-dependent signalling in response to Wnt Reactome pathway (R-HSA-201681). ( B ) Quantification of ADNP protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 4 differentiations). Paired t -test, * P < 0.05. ( C ) Quantification of SOX13 protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 5 differentiations). Paired t -test, ** P < 0.01. ( D ) Schematic of experimental design for overexpression of a gene of interest followed by the SUPERTOPFLASH canonical Wnt reporter assay. ( E ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, ns = not significant. ( F ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( G ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( H ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, *** P < 0.001. ( I ) Genomic structure of SOX13 . Box above gene indicates relative position of gRNA target sequence. Western blot validation of SOX13 knockdown (GAPDH = loading control). ( J ) Gene ontology pathway enrichment results of differential gene expression in SOX13 MUT NPCs, separated by directionality of log 2 fold change. ( K ) GSEA of Wnt-activated targets and G2/M progenitor markers in SOX13 MUT versus SOX13 WT differential expression. ( L ) Model of POU3F2-mediated regulation of canonical Wnt signalling and progenitor expansion via transcription of ADNP and SOX13 . Schematics created in BioRender. Benoit, C. (2025) https://BioRender.com/35q22ly , https://BioRender.com/baxexcu . gRNA = guide RNA; GSEA = gene set enrichment analysis; MUT = mutant; NPC = neural progenitor cell; SEM = standard error of the mean; WT = wild-type.
Sox13, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/islet+cells/SOX13+Antibody/pmc12677912-56-70-71
Average 93 stars, based on 1 article reviews
sox13 - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
islet cells  (AcceGen Biotechnology)
92
AcceGen Biotechnology islet cells
POU3F2 activates expression of <t>SOX13</t> and ADNP to regulate canonical Wnt signalling in NPCs. ( A ) Potential direct effectors of POU3F2 were prioritized if they belonged to the TCF-dependent signalling in response to Wnt Reactome pathway (R-HSA-201681). ( B ) Quantification of ADNP protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 4 differentiations). Paired t -test, * P < 0.05. ( C ) Quantification of SOX13 protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 5 differentiations). Paired t -test, ** P < 0.01. ( D ) Schematic of experimental design for overexpression of a gene of interest followed by the SUPERTOPFLASH canonical Wnt reporter assay. ( E ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, ns = not significant. ( F ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( G ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( H ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, *** P < 0.001. ( I ) Genomic structure of SOX13 . Box above gene indicates relative position of gRNA target sequence. Western blot validation of SOX13 knockdown (GAPDH = loading control). ( J ) Gene ontology pathway enrichment results of differential gene expression in SOX13 MUT NPCs, separated by directionality of log 2 fold change. ( K ) GSEA of Wnt-activated targets and G2/M progenitor markers in SOX13 MUT versus SOX13 WT differential expression. ( L ) Model of POU3F2-mediated regulation of canonical Wnt signalling and progenitor expansion via transcription of ADNP and SOX13 . Schematics created in BioRender. Benoit, C. (2025) https://BioRender.com/35q22ly , https://BioRender.com/baxexcu . gRNA = guide RNA; GSEA = gene set enrichment analysis; MUT = mutant; NPC = neural progenitor cell; SEM = standard error of the mean; WT = wild-type.
Islet Cells, supplied by AcceGen Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/islet+cells/Human+Pancreatic+Islets+of+Langerhans+Cells/pm39627229-407-2-5
Average 92 stars, based on 1 article reviews
islet cells - by Bioz Stars, 2026-09
92/100 stars
  Buy from Supplier
pancreas tissue slides  (Novus Biologicals)
92
Novus Biologicals pancreas tissue slides
POU3F2 activates expression of <t>SOX13</t> and ADNP to regulate canonical Wnt signalling in NPCs. ( A ) Potential direct effectors of POU3F2 were prioritized if they belonged to the TCF-dependent signalling in response to Wnt Reactome pathway (R-HSA-201681). ( B ) Quantification of ADNP protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 4 differentiations). Paired t -test, * P < 0.05. ( C ) Quantification of SOX13 protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 5 differentiations). Paired t -test, ** P < 0.01. ( D ) Schematic of experimental design for overexpression of a gene of interest followed by the SUPERTOPFLASH canonical Wnt reporter assay. ( E ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, ns = not significant. ( F ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( G ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( H ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, *** P < 0.001. ( I ) Genomic structure of SOX13 . Box above gene indicates relative position of gRNA target sequence. Western blot validation of SOX13 knockdown (GAPDH = loading control). ( J ) Gene ontology pathway enrichment results of differential gene expression in SOX13 MUT NPCs, separated by directionality of log 2 fold change. ( K ) GSEA of Wnt-activated targets and G2/M progenitor markers in SOX13 MUT versus SOX13 WT differential expression. ( L ) Model of POU3F2-mediated regulation of canonical Wnt signalling and progenitor expansion via transcription of ADNP and SOX13 . Schematics created in BioRender. Benoit, C. (2025) https://BioRender.com/35q22ly , https://BioRender.com/baxexcu . gRNA = guide RNA; GSEA = gene set enrichment analysis; MUT = mutant; NPC = neural progenitor cell; SEM = standard error of the mean; WT = wild-type.
Pancreas Tissue Slides, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/islet+cells/Pancreas+Tissue+Slides+(Islet+Cell+Tumor)-+Paraffin/bio_rxiv__2022__04__25__489401-98-8-15
Average 92 stars, based on 1 article reviews
pancreas tissue slides - by Bioz Stars, 2026-09
92/100 stars
  Buy from Supplier
novus biologicalstm cat  (Novus Biologicals)
91
Novus Biologicals novus biologicalstm cat
POU3F2 activates expression of <t>SOX13</t> and ADNP to regulate canonical Wnt signalling in NPCs. ( A ) Potential direct effectors of POU3F2 were prioritized if they belonged to the TCF-dependent signalling in response to Wnt Reactome pathway (R-HSA-201681). ( B ) Quantification of ADNP protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 4 differentiations). Paired t -test, * P < 0.05. ( C ) Quantification of SOX13 protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 5 differentiations). Paired t -test, ** P < 0.01. ( D ) Schematic of experimental design for overexpression of a gene of interest followed by the SUPERTOPFLASH canonical Wnt reporter assay. ( E ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, ns = not significant. ( F ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( G ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( H ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, *** P < 0.001. ( I ) Genomic structure of SOX13 . Box above gene indicates relative position of gRNA target sequence. Western blot validation of SOX13 knockdown (GAPDH = loading control). ( J ) Gene ontology pathway enrichment results of differential gene expression in SOX13 MUT NPCs, separated by directionality of log 2 fold change. ( K ) GSEA of Wnt-activated targets and G2/M progenitor markers in SOX13 MUT versus SOX13 WT differential expression. ( L ) Model of POU3F2-mediated regulation of canonical Wnt signalling and progenitor expansion via transcription of ADNP and SOX13 . Schematics created in BioRender. Benoit, C. (2025) https://BioRender.com/35q22ly , https://BioRender.com/baxexcu . gRNA = guide RNA; GSEA = gene set enrichment analysis; MUT = mutant; NPC = neural progenitor cell; SEM = standard error of the mean; WT = wild-type.
Novus Biologicalstm Cat, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/islet+cells/Human+Islet+Cell+Autoantibody+ELISA+Kit+(Colorimetric)/pm37953627-56-14-14
Average 91 stars, based on 1 article reviews
novus biologicalstm cat - by Bioz Stars, 2026-09
91/100 stars
  Buy from Supplier
m35002 04s  (Celprogen Inc)
90
Celprogen Inc m35002 04s
POU3F2 activates expression of <t>SOX13</t> and ADNP to regulate canonical Wnt signalling in NPCs. ( A ) Potential direct effectors of POU3F2 were prioritized if they belonged to the TCF-dependent signalling in response to Wnt Reactome pathway (R-HSA-201681). ( B ) Quantification of ADNP protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 4 differentiations). Paired t -test, * P < 0.05. ( C ) Quantification of SOX13 protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 5 differentiations). Paired t -test, ** P < 0.01. ( D ) Schematic of experimental design for overexpression of a gene of interest followed by the SUPERTOPFLASH canonical Wnt reporter assay. ( E ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, ns = not significant. ( F ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( G ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( H ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, *** P < 0.001. ( I ) Genomic structure of SOX13 . Box above gene indicates relative position of gRNA target sequence. Western blot validation of SOX13 knockdown (GAPDH = loading control). ( J ) Gene ontology pathway enrichment results of differential gene expression in SOX13 MUT NPCs, separated by directionality of log 2 fold change. ( K ) GSEA of Wnt-activated targets and G2/M progenitor markers in SOX13 MUT versus SOX13 WT differential expression. ( L ) Model of POU3F2-mediated regulation of canonical Wnt signalling and progenitor expansion via transcription of ADNP and SOX13 . Schematics created in BioRender. Benoit, C. (2025) https://BioRender.com/35q22ly , https://BioRender.com/baxexcu . gRNA = guide RNA; GSEA = gene set enrichment analysis; MUT = mutant; NPC = neural progenitor cell; SEM = standard error of the mean; WT = wild-type.
M35002 04s, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/islet+cells/Human+Pancreatic+Islets+Of+Langerhan+Cell+Complete+Growth+Media+with+serum/pmc05357144-561-15-14
Average 90 stars, based on 1 article reviews
m35002 04s - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
pancreatic islets human  (Celprogen Inc)
93
Celprogen Inc pancreatic islets human
POU3F2 activates expression of <t>SOX13</t> and ADNP to regulate canonical Wnt signalling in NPCs. ( A ) Potential direct effectors of POU3F2 were prioritized if they belonged to the TCF-dependent signalling in response to Wnt Reactome pathway (R-HSA-201681). ( B ) Quantification of ADNP protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 4 differentiations). Paired t -test, * P < 0.05. ( C ) Quantification of SOX13 protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 5 differentiations). Paired t -test, ** P < 0.01. ( D ) Schematic of experimental design for overexpression of a gene of interest followed by the SUPERTOPFLASH canonical Wnt reporter assay. ( E ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, ns = not significant. ( F ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( G ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( H ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, *** P < 0.001. ( I ) Genomic structure of SOX13 . Box above gene indicates relative position of gRNA target sequence. Western blot validation of SOX13 knockdown (GAPDH = loading control). ( J ) Gene ontology pathway enrichment results of differential gene expression in SOX13 MUT NPCs, separated by directionality of log 2 fold change. ( K ) GSEA of Wnt-activated targets and G2/M progenitor markers in SOX13 MUT versus SOX13 WT differential expression. ( L ) Model of POU3F2-mediated regulation of canonical Wnt signalling and progenitor expansion via transcription of ADNP and SOX13 . Schematics created in BioRender. Benoit, C. (2025) https://BioRender.com/35q22ly , https://BioRender.com/baxexcu . gRNA = guide RNA; GSEA = gene set enrichment analysis; MUT = mutant; NPC = neural progenitor cell; SEM = standard error of the mean; WT = wild-type.
Pancreatic Islets Human, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/islet+cells/Human+Pancreatic+Islets+of+Langerhans+Cell+Culture/pm35499468-41-0-21
Average 93 stars, based on 1 article reviews
pancreatic islets human - by Bioz Stars, 2026-09
93/100 stars
  Buy from Supplier
pre coated flasks  (Celprogen Inc)
90
Celprogen Inc pre coated flasks
POU3F2 activates expression of <t>SOX13</t> and ADNP to regulate canonical Wnt signalling in NPCs. ( A ) Potential direct effectors of POU3F2 were prioritized if they belonged to the TCF-dependent signalling in response to Wnt Reactome pathway (R-HSA-201681). ( B ) Quantification of ADNP protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 4 differentiations). Paired t -test, * P < 0.05. ( C ) Quantification of SOX13 protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 5 differentiations). Paired t -test, ** P < 0.01. ( D ) Schematic of experimental design for overexpression of a gene of interest followed by the SUPERTOPFLASH canonical Wnt reporter assay. ( E ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, ns = not significant. ( F ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( G ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( H ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, *** P < 0.001. ( I ) Genomic structure of SOX13 . Box above gene indicates relative position of gRNA target sequence. Western blot validation of SOX13 knockdown (GAPDH = loading control). ( J ) Gene ontology pathway enrichment results of differential gene expression in SOX13 MUT NPCs, separated by directionality of log 2 fold change. ( K ) GSEA of Wnt-activated targets and G2/M progenitor markers in SOX13 MUT versus SOX13 WT differential expression. ( L ) Model of POU3F2-mediated regulation of canonical Wnt signalling and progenitor expansion via transcription of ADNP and SOX13 . Schematics created in BioRender. Benoit, C. (2025) https://BioRender.com/35q22ly , https://BioRender.com/baxexcu . gRNA = guide RNA; GSEA = gene set enrichment analysis; MUT = mutant; NPC = neural progenitor cell; SEM = standard error of the mean; WT = wild-type.
Pre Coated Flasks, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/islet+cells/Human+Pancreatic+Islets+of+Langerhans+Cell+Culture+Extracellular+Matrix/pm32314809-75-14-16
Average 90 stars, based on 1 article reviews
pre coated flasks - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
pancreatic islets  (AcceGen Biotechnology)
90
AcceGen Biotechnology pancreatic islets
POU3F2 activates expression of <t>SOX13</t> and ADNP to regulate canonical Wnt signalling in NPCs. ( A ) Potential direct effectors of POU3F2 were prioritized if they belonged to the TCF-dependent signalling in response to Wnt Reactome pathway (R-HSA-201681). ( B ) Quantification of ADNP protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 4 differentiations). Paired t -test, * P < 0.05. ( C ) Quantification of SOX13 protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 5 differentiations). Paired t -test, ** P < 0.01. ( D ) Schematic of experimental design for overexpression of a gene of interest followed by the SUPERTOPFLASH canonical Wnt reporter assay. ( E ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, ns = not significant. ( F ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( G ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( H ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, *** P < 0.001. ( I ) Genomic structure of SOX13 . Box above gene indicates relative position of gRNA target sequence. Western blot validation of SOX13 knockdown (GAPDH = loading control). ( J ) Gene ontology pathway enrichment results of differential gene expression in SOX13 MUT NPCs, separated by directionality of log 2 fold change. ( K ) GSEA of Wnt-activated targets and G2/M progenitor markers in SOX13 MUT versus SOX13 WT differential expression. ( L ) Model of POU3F2-mediated regulation of canonical Wnt signalling and progenitor expansion via transcription of ADNP and SOX13 . Schematics created in BioRender. Benoit, C. (2025) https://BioRender.com/35q22ly , https://BioRender.com/baxexcu . gRNA = guide RNA; GSEA = gene set enrichment analysis; MUT = mutant; NPC = neural progenitor cell; SEM = standard error of the mean; WT = wild-type.
Pancreatic Islets, supplied by AcceGen Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/islet+cells/Human+Pancreatic+Islet+Beta+Cells/pmc08268282-185-2-11
Average 90 stars, based on 1 article reviews
pancreatic islets - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
pancreatic islets  (Celprogen Inc)
90
Celprogen Inc pancreatic islets
POU3F2 activates expression of <t>SOX13</t> and ADNP to regulate canonical Wnt signalling in NPCs. ( A ) Potential direct effectors of POU3F2 were prioritized if they belonged to the TCF-dependent signalling in response to Wnt Reactome pathway (R-HSA-201681). ( B ) Quantification of ADNP protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 4 differentiations). Paired t -test, * P < 0.05. ( C ) Quantification of SOX13 protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 5 differentiations). Paired t -test, ** P < 0.01. ( D ) Schematic of experimental design for overexpression of a gene of interest followed by the SUPERTOPFLASH canonical Wnt reporter assay. ( E ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, ns = not significant. ( F ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( G ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( H ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, *** P < 0.001. ( I ) Genomic structure of SOX13 . Box above gene indicates relative position of gRNA target sequence. Western blot validation of SOX13 knockdown (GAPDH = loading control). ( J ) Gene ontology pathway enrichment results of differential gene expression in SOX13 MUT NPCs, separated by directionality of log 2 fold change. ( K ) GSEA of Wnt-activated targets and G2/M progenitor markers in SOX13 MUT versus SOX13 WT differential expression. ( L ) Model of POU3F2-mediated regulation of canonical Wnt signalling and progenitor expansion via transcription of ADNP and SOX13 . Schematics created in BioRender. Benoit, C. (2025) https://BioRender.com/35q22ly , https://BioRender.com/baxexcu . gRNA = guide RNA; GSEA = gene set enrichment analysis; MUT = mutant; NPC = neural progenitor cell; SEM = standard error of the mean; WT = wild-type.
Pancreatic Islets, supplied by Celprogen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/islet+cells/Human+Pancreatic+Islets+of+Langerhans+Cell+Culture+Complete+Growth+Serum+Free+Media/pm32314809-78-15-24
Average 90 stars, based on 1 article reviews
pancreatic islets - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
immunosorbent antibody assay elisa kit  (Cusabio)
86
Cusabio immunosorbent antibody assay elisa kit
POU3F2 activates expression of <t>SOX13</t> and ADNP to regulate canonical Wnt signalling in NPCs. ( A ) Potential direct effectors of POU3F2 were prioritized if they belonged to the TCF-dependent signalling in response to Wnt Reactome pathway (R-HSA-201681). ( B ) Quantification of ADNP protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 4 differentiations). Paired t -test, * P < 0.05. ( C ) Quantification of SOX13 protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 5 differentiations). Paired t -test, ** P < 0.01. ( D ) Schematic of experimental design for overexpression of a gene of interest followed by the SUPERTOPFLASH canonical Wnt reporter assay. ( E ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, ns = not significant. ( F ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( G ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( H ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, *** P < 0.001. ( I ) Genomic structure of SOX13 . Box above gene indicates relative position of gRNA target sequence. Western blot validation of SOX13 knockdown (GAPDH = loading control). ( J ) Gene ontology pathway enrichment results of differential gene expression in SOX13 MUT NPCs, separated by directionality of log 2 fold change. ( K ) GSEA of Wnt-activated targets and G2/M progenitor markers in SOX13 MUT versus SOX13 WT differential expression. ( L ) Model of POU3F2-mediated regulation of canonical Wnt signalling and progenitor expansion via transcription of ADNP and SOX13 . Schematics created in BioRender. Benoit, C. (2025) https://BioRender.com/35q22ly , https://BioRender.com/baxexcu . gRNA = guide RNA; GSEA = gene set enrichment analysis; MUT = mutant; NPC = neural progenitor cell; SEM = standard error of the mean; WT = wild-type.
Immunosorbent Antibody Assay Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/islet+cells/Mouse+islet+cell+antibody%2CICA+ELISA+Kit/pm29788452-59-15-20
Average 86 stars, based on 1 article reviews
immunosorbent antibody assay elisa kit - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier
islet equivalent cells  (ViaCyte Inc)
90
ViaCyte Inc islet equivalent cells
POU3F2 activates expression of <t>SOX13</t> and ADNP to regulate canonical Wnt signalling in NPCs. ( A ) Potential direct effectors of POU3F2 were prioritized if they belonged to the TCF-dependent signalling in response to Wnt Reactome pathway (R-HSA-201681). ( B ) Quantification of ADNP protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 4 differentiations). Paired t -test, * P < 0.05. ( C ) Quantification of SOX13 protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 5 differentiations). Paired t -test, ** P < 0.01. ( D ) Schematic of experimental design for overexpression of a gene of interest followed by the SUPERTOPFLASH canonical Wnt reporter assay. ( E ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, ns = not significant. ( F ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( G ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( H ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, *** P < 0.001. ( I ) Genomic structure of SOX13 . Box above gene indicates relative position of gRNA target sequence. Western blot validation of SOX13 knockdown (GAPDH = loading control). ( J ) Gene ontology pathway enrichment results of differential gene expression in SOX13 MUT NPCs, separated by directionality of log 2 fold change. ( K ) GSEA of Wnt-activated targets and G2/M progenitor markers in SOX13 MUT versus SOX13 WT differential expression. ( L ) Model of POU3F2-mediated regulation of canonical Wnt signalling and progenitor expansion via transcription of ADNP and SOX13 . Schematics created in BioRender. Benoit, C. (2025) https://BioRender.com/35q22ly , https://BioRender.com/baxexcu . gRNA = guide RNA; GSEA = gene set enrichment analysis; MUT = mutant; NPC = neural progenitor cell; SEM = standard error of the mean; WT = wild-type.
Islet Equivalent Cells, supplied by ViaCyte Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/islet+cells/islet+equivalent+cells/pmc03412316-136-23-6
Average 90 stars, based on 1 article reviews
islet equivalent cells - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


POU3F2 activates expression of SOX13 and ADNP to regulate canonical Wnt signalling in NPCs. ( A ) Potential direct effectors of POU3F2 were prioritized if they belonged to the TCF-dependent signalling in response to Wnt Reactome pathway (R-HSA-201681). ( B ) Quantification of ADNP protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 4 differentiations). Paired t -test, * P < 0.05. ( C ) Quantification of SOX13 protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 5 differentiations). Paired t -test, ** P < 0.01. ( D ) Schematic of experimental design for overexpression of a gene of interest followed by the SUPERTOPFLASH canonical Wnt reporter assay. ( E ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, ns = not significant. ( F ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( G ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( H ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, *** P < 0.001. ( I ) Genomic structure of SOX13 . Box above gene indicates relative position of gRNA target sequence. Western blot validation of SOX13 knockdown (GAPDH = loading control). ( J ) Gene ontology pathway enrichment results of differential gene expression in SOX13 MUT NPCs, separated by directionality of log 2 fold change. ( K ) GSEA of Wnt-activated targets and G2/M progenitor markers in SOX13 MUT versus SOX13 WT differential expression. ( L ) Model of POU3F2-mediated regulation of canonical Wnt signalling and progenitor expansion via transcription of ADNP and SOX13 . Schematics created in BioRender. Benoit, C. (2025) https://BioRender.com/35q22ly , https://BioRender.com/baxexcu . gRNA = guide RNA; GSEA = gene set enrichment analysis; MUT = mutant; NPC = neural progenitor cell; SEM = standard error of the mean; WT = wild-type.

Journal: Brain

Article Title: POU3F2 regulates canonical Wnt signalling via SOX13 and ADNP to expand the neural progenitor population

doi: 10.1093/brain/awaf221

Figure Lengend Snippet: POU3F2 activates expression of SOX13 and ADNP to regulate canonical Wnt signalling in NPCs. ( A ) Potential direct effectors of POU3F2 were prioritized if they belonged to the TCF-dependent signalling in response to Wnt Reactome pathway (R-HSA-201681). ( B ) Quantification of ADNP protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 4 differentiations). Paired t -test, * P < 0.05. ( C ) Quantification of SOX13 protein expression measured via western blotting in POU3F2 WT and POU3F2 MUT NPCs, mean ± SEM ( n = 5 differentiations). Paired t -test, ** P < 0.01. ( D ) Schematic of experimental design for overexpression of a gene of interest followed by the SUPERTOPFLASH canonical Wnt reporter assay. ( E ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, ns = not significant. ( F ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or ADNP, mean ± SEM ( n = 3 differentiations). Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( G ) Baseline canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, ** P < 0.01. ( H ) Activated canonical Wnt signalling, as measured by a SUPERTOPFLASH assay, in POU3F2 WT and POU3F2 MUT NPCs expressing GFP (negative control) or SOX13, mean ± SEM ( n = 3 differentiations). GFP control data represented here is included in . Mixed-effects model with post hoc Sidak's test, * P < 0.05, *** P < 0.001. ( I ) Genomic structure of SOX13 . Box above gene indicates relative position of gRNA target sequence. Western blot validation of SOX13 knockdown (GAPDH = loading control). ( J ) Gene ontology pathway enrichment results of differential gene expression in SOX13 MUT NPCs, separated by directionality of log 2 fold change. ( K ) GSEA of Wnt-activated targets and G2/M progenitor markers in SOX13 MUT versus SOX13 WT differential expression. ( L ) Model of POU3F2-mediated regulation of canonical Wnt signalling and progenitor expansion via transcription of ADNP and SOX13 . Schematics created in BioRender. Benoit, C. (2025) https://BioRender.com/35q22ly , https://BioRender.com/baxexcu . gRNA = guide RNA; GSEA = gene set enrichment analysis; MUT = mutant; NPC = neural progenitor cell; SEM = standard error of the mean; WT = wild-type.

Article Snippet: Cells were incubated with primary antibody diluted in blocking buffer overnight at 4°C: POU3F2 (CST, Cat. No. 12137S, 1:100), Nestin (Novus Biologicals, Cat. No. MAB1259, 1:500), PAX6 (Biolegend, Cat. No. 901301, 1:400), SOX2 (R&D Systems, Cat. No. AF2018, 1:500), MAP2 (Abcam, Cat. No. ab5392, 1:500), CD44 (Abcam, Cat. No. ab254530, 1:500), ANXA1 (Abcam, Cat. No. 214486, 1:100), BLBP (R&D Systems, Cat. No. AF3166, 1:100), ADNP (Abcam, Cat. No. ab300114, 1:100), SOX13 (Proteintech, Cat. No. 18902-1, 1:100), β-catenin (CST, Cat. No. 8480S, 1:100).

Techniques: Expressing, Western Blot, Over Expression, Reporter Assay, Negative Control, Control, Sequencing, Biomarker Discovery, Knockdown, Gene Expression, Quantitative Proteomics, Mutagenesis